Office of Academic Affairs
Indian Institute of Science Education and Research Berhampur

Biological Sciences

BIO 204:General Biology Laboratory IV (Credit, 1)

Course Instructors: Prabhat Singh

Course Contents:

Competent cell preparation, Transformation, Plasmid DNA isolation, Agarose gel electrophoresis, Genomic DNA isolation (from E.coli), Nucleic acid estimation, Restriction mapping, Polymerase chain reaction, SDS- PAGE, Genomic DNA isolation (from Yeast)

Suggested Reading: Sambrook and Russel, Molecular cloning: A Laboratory Manual:


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