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Indian Institute of Science Education and Research Berhampur

BIO 308:  Biology Laboratory II (Credit, 3)

Course Instructors:: Prabhat Singh, R Selvi Bharathavikru, Prof. Yogendra Sharma

Course Contents:Biochemistry I, Animal Physiology, Molecular Biology

• Alkaline Phosphatase Assay (Enzyme kinetics)
• Salivary Amylase Assay
• Total Glutathione Estimation
• Glutathione Peroxidase Assay (Enzyme kinetics)
• Plasmid isolation, RE digestion, Molecular Cloning (Cloning revisited, DNA isolation from plant (CTAB Protocol), PCR, Gel Analysis and Gel extraction, Ligation and Transformation, Colony PCR, Patch to new plates, Plasmid Prep, Restriction Analysis
• Determination of Blood parameters (Blood Grouping, Blood smear preparation, Haemoglobin Estimation, WBC & RBC Count)

Suggested Books:Wilson and Walker

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