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Indian Institute of Science Education and Research Berhampur

BIO 416:Biology Laboratory IV (Credit, 3)

Course Instructors:Prabhat Singh, Vinay Bulusu, R Selvi Bharathavikru

Course Content:Dev Biology, Techniques in Bio,

• Cuticle Mount to observe Denticular bands in Drosophila embryos or first instar larva
• Reporter Gene Assay, B-gal staining for wingless expression (might replace with in situ)
• Chick embryo staging
• RNA isolation, Semi quantitative PCR
• qRT-PCR
• Tutorial on Gene Expression Analysis
• Spectroscopy

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