Office of Academic Affairs
Indian Institute of Science Education and Research Berhampur

BIO 417: Fundamentals of NMR Spectroscopy (OE, Credit, 3)

Course Content:

• Basic Concepts, Theory and Applications of NMR Spectroscopy
• Characteristics of Nuclear Spins in NMR
• Concept of Nuclear Spin States
• Generation of a FID in NMR
• Fourier Transform NMR Spectroscopy
• The Concept of Fourier Transform
• The Origin of Chemical Shifts in NMR Spectroscopy
• Inductive effects
• Quantum Mechanical Model for Spin 1/2 Nuclei
• After application of an RF pulse
• Relaxation Processes
• Shell Model Energy Diagram for various spins
• Quadrature detection
• Analog to Digital Conversion
• AB, AMX, and AX spin systems
• Multi-pulse Experiments
• Inversion Recovery Experiment
• Spin-echo pulse sequence
• Spin polarization
• Selective Polarization Transfer
• Selective Population Inversion
• Heteronuclear Selective Population Inversion
• Pre-saturation
• Jump and Return Sequence
• Spectra on a 500 MHz NMR spectrometer
• NMR spectra of amino Acids
• Introduction to two-dimensional NMR
• 2D COSY
• 2D Relayed-COSY
• Multiple-quantum Filtered COSY
• 2D NOESY
• 2D Heteronuclear COSY
• Introduction to 3D NMR
• Triple resonance spectra
• Application to the study of the structure and dynamics of biomolecules.

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