Office of Academic Affairs
Indian Institute of Science Education and Research Berhampur

BIO 424: Techniques in Biology (Credit, 4)

Course Content:

1. Genes and genomes:
• Gene identification and genome assembly:
• Sequencing techniques:
• DNA library prep:
• NGS (different platforms):
• Genome editing CRISPR, TALENS, Z-Fingers:
• QTL Analysis:
• GWAS:
• Marker assisted Selection (MAS):
2. Genetics
3. Gene expression
• RNA In situ & FISH:
• qPCR:
• ChIP & ChIPseq:
• Ribosome profiling:
• Northern & Southern blotting:
• Microarray:
• RNA seq & Introduction to data analysis:
• Reporter Assays:
• RNAi & Artificial MicroRNA:
4. Cell biology
• FACS:
• Imaging (confocal, FLIM, FRET, FRAP etc):
5. Protein Sequence, Structure, Function, interaction
• Protein purification, Protein Analysis (2DGE, Western blotting):
• Optical methods for protein interaction analysis (ITC):
• Hydrodynamic properties (DLS):
• ITC
• DSC:
• Phosphoproteomics:
• Protein Array
6. Molecular Spectroscopy
7. Absorbance and fluorescence spectroscopy, Beer-Lambert’s law, Chromophore, Fluorophore.
8. Mass spectrometry, basics, design principles.
9. Elemental metabolomics-qualitative and quantitative
10. Protein analysis by mass spectrometry- Mass and PTM analysis, Proteomics.

Suggested Books:

Sambrook and Russel, Molecular cloning: A Laboratory Manual
Principles and Techniques of Biochemistry and Molecular Biology, Wilson and Walker

Previous

Back to Course List

Next