Office of Academic Affairs
Indian Institute of Science Education and Research Berhampur


CHM 626: Physical Photochemistry (4)

Prerequisites: CHM 422/622

Introduction to absorption: Lambert-Beer law and its deviation relation between molar extinction coefficient and absorption cross section, Einstein induced absorption coefficient and integrated Einstein induced coefficients, notation of energy levels and electronic transitions

Fluorescence: introduction, Jablonski diagram, kinetic parameters, Einstein’s induced and spontaneous emission coefficients, relationship between lifetime and Einstein coefficients (Strickler and Berg’ equation) and its limitations, fluorescence quantum yield, Stoke’s shift, fluorescence excitation spectrum

Effects of solvents on the fluorescence spectrum: (general effects and specific effects, derivation of the equation), time scales of molecular processes in solutions, applications

Fluorescence quenching: Different mechanism of fluorescence quenching, applications

Radiationless processes: Mechanism for internal and intersystem crossing, effect of temperature of radiationless processes

Phosphorescence: kinetic parameters, origin of triplet state and its formation, different methods of triplet-triplet absorption Fluorescence Anisotropy and its applications

Resonance Energy Transfer: Different mechanisms of energy transfer (Forster and Dexter mechanism), selection rules for energy transfer, non-vertical energy transfer, Forster Resonance Energy Transfer (FRET), typical examples and choice of dyes

Spectrophotometry and Fluorometry: principles and instrumentation; choice of light sources, monochromators, choice of optical filters and various detector systems used, Concept of Time Correlated Single Photon Counting: Basic principles and instrumentation

Fluorophores and dyes used in spectroscopy: intrinsic and extrinsic fluorophores, protein labeling.

Suggested Readings :

Previous Back to Course List Next