Fast Performance Liquid Chromatography (FPLC) System:

Kugelrohr Short Path Distillation Unit
The ÄKTA pure 25 M is a Fast Performance Liquid Chromatography system for automated purification of proteins, peptides, and nucleic acids from microgram to gram levels. Experiments based on various chromatographic techniques, viz., affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC) can be performed on this system for purification and analytical purposes. The FPLC unit is enclosed in a cold cabinet in the interest of temperature sensitive experiments.

Make & Model : Wipro GE Healthcare Pvt. Ltd., Akta Pure 25m
Installation Date : 02.07.2019
Current Location : Room no. – 04, Ground floor

Important Features of the System:

  1. The number of inlets: 7 in pump A and 1 in pump B
  2. Range of the flow rate: 0.001 to 25 ml/min
  3. Max. pressure: 20 MPa.
  4. Monitoring Wavelengths: Xe flash lamp detects at up to 3 wavelengths simultaneously, in the range of 190-700 nm.
  5. Online pH Monitoring.
  6. Conductivity monitoring range: 0.01 mS/cm - 999 mS/cm.
  7. The column control device can switch the direction of flow in column and also bypass the column entirely.
  8. Air sensors in the inlet and before the column can detect the presence of air in the mobile phase.

This Section will be updated soon.

Email: caif@iiserbpr.ac.in