Isothermal Titration Calorimetry (ITC) System:

Isothermal Titration Calorimetry (ITC) System
The Isothermal Titration Calorimetry (ITC) technique is used in the study of molecular interactions. It can directly measure the heat released or absorbed during a binding event, and facilitates direct, label free and in-solution measurements of binding parameters like stoichiometry, binding constant, and changes in enthalpy and entropy through a single experiment. This system works with high sensitivity and requires low quantity of sample. It also has automated washing modules for cleaning of cell and syringe.

Make & Model : Malvern Panalytical Limited ,MICROCAL PEAQ-ITC.
Installation Date : 11.07.19
Current Location : Room no. – 04, Ground floor

Important Features of the System:

  1. Sample volume required: 280 μl.
  2. Sensitivity: Minimum 10 μg protein can be detected.
  3. Range of measured affinities (KD): 10-2 M to 10-9 M.
  4. Cell material: hastelloy.
  5. The analysis software supports many automated fitting models:
    1. One Set of Sites.
    2. Sequential Binding Sites.
    3. One Set of Sites - SIM.
    4. Two Sets of Sites.

This Section will be updated soon.