Isothermal Titration Calorimetry (ITC) System:

The Isothermal Titration Calorimetry (ITC) technique is used in the study of molecular interactions. It can directly measure the heat released or absorbed during a binding event, and facilitates direct, label free and in-solution measurements of binding parameters like stoichiometry, binding constant, and changes in enthalpy and entropy through a single experiment. This system works with high sensitivity and requires low quantity of sample. It also has automated washing modules for cleaning of cell and syringe.
Make & Model : Malvern Panalytical Limited ,MICROCAL PEAQ-ITC.
Installation Date : 11.07.19
Current Location : Room no. – 04, Ground floor
Important Features of the System:
- Sample volume required: 280 μl.
- Sensitivity: Minimum 10 μg protein can be detected.
- Range of measured affinities (KD): 10-2 M to 10-9 M.
- Cell material: hastelloy.
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The analysis software supports many automated fitting models:
- One Set of Sites.
- Sequential Binding Sites.
- One Set of Sites - SIM.
- Two Sets of Sites.
This Section will be updated soon.
Email: caif@iiserbpr.ac.in